Search results for " Elisa"
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Make the Switch from ELISA to Biacore™ SPR-Based Assays Today
General guidance on how to convert an existing ELISA assay to a Biacore surface plasmon resonance (SPR) assay and how, by doing so, you can get results in less than half the time compared with ELISA. …
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Host cell protein quantitation using ELISA
This application note shows how using the sensitive HCPQuant™ CHO ELISA kit (Cytiva) and automating wash steps using Aquamax 4000 Microplater Washer (Molecular Devices) enables fast results without co…
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Why, Why, Why…ELISA? A Look at the Benchmark HCP Assay
Let’s take a close look at why analytical scientists turn to the ELISA and how it fits into process development.
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News: Cytiva Adds ELISA Kit to End-to-End HCP Monitoring Capabilities
Cytiva is pleased to announce the new Amersham HCPQuant CHO kit, a generic for detecting host cell protein impurities in drug samples produced in CHO cell lines.
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Host-Cell Protein Measurement and Control
Using these polyclonal antibodies, a multi-analyte sandwich ELISA can be developed (see Figure 1). In Figure 1, capture antibodies enrich the HCPs from sample and immobilize them to a 96 (or greater)-…
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Emerging Therapies Test Existing Bioanalytical Methods
…ese characteristics are often not traditional liquid chromatography–mass spectrometry (LC–MS) or ELISA used to quantify small- and large-molecule therapeutics and biomarkers. The May 2018 FDA Bioanal…
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Impurity Testing of Biologic Drug Products
Process-related impurities and residuals such as Protein A can be detected and quantified with ELISA assays, whereas host cell residual DNA can be quantified via quantitative polymerase chain reaction…
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Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
SPR and ELISA are extensively used in ligand-binding assays. SPR analysis has been used for antibody characterization for more than 20 years. The sample readout from an SPR system such as Biacore (Cyt…
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Ligand-Binding Assays and the Determination of Biosimilarity
LBA detection techniques
There are essentially two general methods for assessing ligand binding: ELISA (enzyme-linked immunosorbent assay)-type assays and kinetic binding assays. Classical ELISA a…
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Virus-like Particles as Therapeutic Moieties of the Future
Solution competitive ELISA tracks the correct display of epitopes on VLPs with a neutralizing monoclonal antibody against the respective epitope, which allows real-time monitoring of relative antigeni…